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nox4  (Novus Biologicals)


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    Structured Review

    Novus Biologicals nox4
    PM2.5 nasal administration increases protein and mRNA levels of <t>NOX4</t> in the hypothalamus but not in the hippocampus. ( A , D ) Western blot analysis of <t>NOX4</t> <t>protein</t> levels in the hypothalamus ( A ) and hippocampus ( D ) following vehicle or PM2.5 treatment. β-actin served as a loading control. ( B , E ) Quantification of NOX4 protein levels from panels A and D, respectively. ( C , F ) Real-time qPCR for NOX4 mRNA level in the ( C ) hypothalamus and ( F ) hippocampus following vehicle or PM2.5 treatment. Data are presented as mean ± SEM, with comparisons made using Student’s t -test in panels ( B , D ). ns., not significant. * p ≤ 0.05 vs. Vehicle, n = 4.
    Nox4, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nox4/product/Novus Biologicals
    Average 93 stars, based on 69 article reviews
    nox4 - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "PM2.5 Exposure Triggers Hypothalamic Oxidative and ER Stress Leading to Depressive-like Behaviors in Rats"

    Article Title: PM2.5 Exposure Triggers Hypothalamic Oxidative and ER Stress Leading to Depressive-like Behaviors in Rats

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms252413527

    PM2.5 nasal administration increases protein and mRNA levels of NOX4 in the hypothalamus but not in the hippocampus. ( A , D ) Western blot analysis of NOX4 protein levels in the hypothalamus ( A ) and hippocampus ( D ) following vehicle or PM2.5 treatment. β-actin served as a loading control. ( B , E ) Quantification of NOX4 protein levels from panels A and D, respectively. ( C , F ) Real-time qPCR for NOX4 mRNA level in the ( C ) hypothalamus and ( F ) hippocampus following vehicle or PM2.5 treatment. Data are presented as mean ± SEM, with comparisons made using Student’s t -test in panels ( B , D ). ns., not significant. * p ≤ 0.05 vs. Vehicle, n = 4.
    Figure Legend Snippet: PM2.5 nasal administration increases protein and mRNA levels of NOX4 in the hypothalamus but not in the hippocampus. ( A , D ) Western blot analysis of NOX4 protein levels in the hypothalamus ( A ) and hippocampus ( D ) following vehicle or PM2.5 treatment. β-actin served as a loading control. ( B , E ) Quantification of NOX4 protein levels from panels A and D, respectively. ( C , F ) Real-time qPCR for NOX4 mRNA level in the ( C ) hypothalamus and ( F ) hippocampus following vehicle or PM2.5 treatment. Data are presented as mean ± SEM, with comparisons made using Student’s t -test in panels ( B , D ). ns., not significant. * p ≤ 0.05 vs. Vehicle, n = 4.

    Techniques Used: Western Blot, Control

    PM2.5 induces ER stress and mitochondrial ROS production, reducing neuronal markers in Neuro-2A cells. ( A ) q-PCR analysis of ER stress markers ATF4 and CHOP in Neuro-2A cells treated with low (1 μg/mL) and high (100 μg/mL) doses of PM2.5. ( B ) Western blot analysis of NOX4 protein levels in Neuro-2A cells treated with vehicle, 1 μg/mL, and 100 μg/mL of PM2.5, with β-actin as a loading control. ( C , D ) Mitochondrial ROS production in Neuro-2A cells treated with PM2.5 (100 μg/mL), showing a significant increase in ROS levels compared to vehicle control. ( E , F ) PM2.5-induced mitochondrial ROS increase was significantly reduced by NOX inhibitors GKT137831 and Apocynin. ( G ) Western blot analysis of β-III-tubulin and VAChT protein levels in Neuro-2A cells treated with increasing concentrations of PM2.5 (1, 3, 10, 30, and 100 μg/mL), with β-actin as a loading control. ( H , I ) Quantifying β-III-tubulin ( H ) and VAChT ( I ) protein levels from panel G, respectively. Scale bars, 5 μm. Bar graphs are expressed as mean ± SEM and analyzed using one-way ANOVA ( A , D , F , H , I ). * p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.001 vs. Vehicle. # p ≤ 0.05 vs. PM2.5.
    Figure Legend Snippet: PM2.5 induces ER stress and mitochondrial ROS production, reducing neuronal markers in Neuro-2A cells. ( A ) q-PCR analysis of ER stress markers ATF4 and CHOP in Neuro-2A cells treated with low (1 μg/mL) and high (100 μg/mL) doses of PM2.5. ( B ) Western blot analysis of NOX4 protein levels in Neuro-2A cells treated with vehicle, 1 μg/mL, and 100 μg/mL of PM2.5, with β-actin as a loading control. ( C , D ) Mitochondrial ROS production in Neuro-2A cells treated with PM2.5 (100 μg/mL), showing a significant increase in ROS levels compared to vehicle control. ( E , F ) PM2.5-induced mitochondrial ROS increase was significantly reduced by NOX inhibitors GKT137831 and Apocynin. ( G ) Western blot analysis of β-III-tubulin and VAChT protein levels in Neuro-2A cells treated with increasing concentrations of PM2.5 (1, 3, 10, 30, and 100 μg/mL), with β-actin as a loading control. ( H , I ) Quantifying β-III-tubulin ( H ) and VAChT ( I ) protein levels from panel G, respectively. Scale bars, 5 μm. Bar graphs are expressed as mean ± SEM and analyzed using one-way ANOVA ( A , D , F , H , I ). * p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.001 vs. Vehicle. # p ≤ 0.05 vs. PM2.5.

    Techniques Used: Western Blot, Control



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    nox4  (Novus Biologicals)
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    Novus Biologicals nox4
    PM2.5 nasal administration increases protein and mRNA levels of <t>NOX4</t> in the hypothalamus but not in the hippocampus. ( A , D ) Western blot analysis of <t>NOX4</t> <t>protein</t> levels in the hypothalamus ( A ) and hippocampus ( D ) following vehicle or PM2.5 treatment. β-actin served as a loading control. ( B , E ) Quantification of NOX4 protein levels from panels A and D, respectively. ( C , F ) Real-time qPCR for NOX4 mRNA level in the ( C ) hypothalamus and ( F ) hippocampus following vehicle or PM2.5 treatment. Data are presented as mean ± SEM, with comparisons made using Student’s t -test in panels ( B , D ). ns., not significant. * p ≤ 0.05 vs. Vehicle, n = 4.
    Nox4, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nox4/product/Novus Biologicals
    Average 93 stars, based on 1 article reviews
    nox4 - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    PM2.5 nasal administration increases protein and mRNA levels of NOX4 in the hypothalamus but not in the hippocampus. ( A , D ) Western blot analysis of NOX4 protein levels in the hypothalamus ( A ) and hippocampus ( D ) following vehicle or PM2.5 treatment. β-actin served as a loading control. ( B , E ) Quantification of NOX4 protein levels from panels A and D, respectively. ( C , F ) Real-time qPCR for NOX4 mRNA level in the ( C ) hypothalamus and ( F ) hippocampus following vehicle or PM2.5 treatment. Data are presented as mean ± SEM, with comparisons made using Student’s t -test in panels ( B , D ). ns., not significant. * p ≤ 0.05 vs. Vehicle, n = 4.

    Journal: International Journal of Molecular Sciences

    Article Title: PM2.5 Exposure Triggers Hypothalamic Oxidative and ER Stress Leading to Depressive-like Behaviors in Rats

    doi: 10.3390/ijms252413527

    Figure Lengend Snippet: PM2.5 nasal administration increases protein and mRNA levels of NOX4 in the hypothalamus but not in the hippocampus. ( A , D ) Western blot analysis of NOX4 protein levels in the hypothalamus ( A ) and hippocampus ( D ) following vehicle or PM2.5 treatment. β-actin served as a loading control. ( B , E ) Quantification of NOX4 protein levels from panels A and D, respectively. ( C , F ) Real-time qPCR for NOX4 mRNA level in the ( C ) hypothalamus and ( F ) hippocampus following vehicle or PM2.5 treatment. Data are presented as mean ± SEM, with comparisons made using Student’s t -test in panels ( B , D ). ns., not significant. * p ≤ 0.05 vs. Vehicle, n = 4.

    Article Snippet: The primary antibodies used for immunoblotting were: GAPDH (diluted 1:2500, sc-365062) and TH (diluted 1:1000, sc-25269) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); VAChT (diluted 1:1000, #75-020) from NeuroMab Facility (UC Davis/NIH, CA, USA); β-actin (diluted 1:2500, ab6276) and β-III-tubulin (#ab7751, diluted 1:1000) from Abcam (Cambridge, UK); NOX4 (diluted 1:1000, NB110-58849) from Novus Biologicals (Littleton, CO, USA).

    Techniques: Western Blot, Control

    PM2.5 induces ER stress and mitochondrial ROS production, reducing neuronal markers in Neuro-2A cells. ( A ) q-PCR analysis of ER stress markers ATF4 and CHOP in Neuro-2A cells treated with low (1 μg/mL) and high (100 μg/mL) doses of PM2.5. ( B ) Western blot analysis of NOX4 protein levels in Neuro-2A cells treated with vehicle, 1 μg/mL, and 100 μg/mL of PM2.5, with β-actin as a loading control. ( C , D ) Mitochondrial ROS production in Neuro-2A cells treated with PM2.5 (100 μg/mL), showing a significant increase in ROS levels compared to vehicle control. ( E , F ) PM2.5-induced mitochondrial ROS increase was significantly reduced by NOX inhibitors GKT137831 and Apocynin. ( G ) Western blot analysis of β-III-tubulin and VAChT protein levels in Neuro-2A cells treated with increasing concentrations of PM2.5 (1, 3, 10, 30, and 100 μg/mL), with β-actin as a loading control. ( H , I ) Quantifying β-III-tubulin ( H ) and VAChT ( I ) protein levels from panel G, respectively. Scale bars, 5 μm. Bar graphs are expressed as mean ± SEM and analyzed using one-way ANOVA ( A , D , F , H , I ). * p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.001 vs. Vehicle. # p ≤ 0.05 vs. PM2.5.

    Journal: International Journal of Molecular Sciences

    Article Title: PM2.5 Exposure Triggers Hypothalamic Oxidative and ER Stress Leading to Depressive-like Behaviors in Rats

    doi: 10.3390/ijms252413527

    Figure Lengend Snippet: PM2.5 induces ER stress and mitochondrial ROS production, reducing neuronal markers in Neuro-2A cells. ( A ) q-PCR analysis of ER stress markers ATF4 and CHOP in Neuro-2A cells treated with low (1 μg/mL) and high (100 μg/mL) doses of PM2.5. ( B ) Western blot analysis of NOX4 protein levels in Neuro-2A cells treated with vehicle, 1 μg/mL, and 100 μg/mL of PM2.5, with β-actin as a loading control. ( C , D ) Mitochondrial ROS production in Neuro-2A cells treated with PM2.5 (100 μg/mL), showing a significant increase in ROS levels compared to vehicle control. ( E , F ) PM2.5-induced mitochondrial ROS increase was significantly reduced by NOX inhibitors GKT137831 and Apocynin. ( G ) Western blot analysis of β-III-tubulin and VAChT protein levels in Neuro-2A cells treated with increasing concentrations of PM2.5 (1, 3, 10, 30, and 100 μg/mL), with β-actin as a loading control. ( H , I ) Quantifying β-III-tubulin ( H ) and VAChT ( I ) protein levels from panel G, respectively. Scale bars, 5 μm. Bar graphs are expressed as mean ± SEM and analyzed using one-way ANOVA ( A , D , F , H , I ). * p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.001 vs. Vehicle. # p ≤ 0.05 vs. PM2.5.

    Article Snippet: The primary antibodies used for immunoblotting were: GAPDH (diluted 1:2500, sc-365062) and TH (diluted 1:1000, sc-25269) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); VAChT (diluted 1:1000, #75-020) from NeuroMab Facility (UC Davis/NIH, CA, USA); β-actin (diluted 1:2500, ab6276) and β-III-tubulin (#ab7751, diluted 1:1000) from Abcam (Cambridge, UK); NOX4 (diluted 1:1000, NB110-58849) from Novus Biologicals (Littleton, CO, USA).

    Techniques: Western Blot, Control